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Inovativní metody v chovu a reprodukci candáta obecného (Sander lucioperca)
BLECHA, Miroslav
The whole Ph.D. thesis includes in total 10 chapters on 146 pages. Chapter 2 9 are specific parts of thesis where following scientific and practical aspects of pikeperch reproduction biology and aquaculture are described. Quality and quantity of pikeperch spermatozoa after varying cold water treatments are presented in the chapter 2 and can help to optimize broodstock management of males with the aim to obtain high quality spermatozoa during a seasonal and an out of season spawning as well. Benefits of hormone treatment of both sexes in semi-artificial reproduction in pikeperch are described in the chapter 3 where the importance of hormonal treatment of both sexes in tank spawning is evaluated for effective production pikeperch larvae. The use of an alcalase treatment for the elimination of pikeperch egg stickiness is being shown in the chapter 4 of this thesis. Post-ovulatory oocyte ageing and its effect on eggs viability rates and occurrence of larval malformations and ploidy anomalies are listed in chapter 5. These results describe the effects of the egg over ripening fertilization process in pikeperch. The first report of heat shock triploidisation in pikeperch is described in chapter 6 with production of 100% pikeperch triploid population. The effect of water surface treatment on survival, swim bladder inflation and growth of larvae is given in the chapter 7 with the aim to optimize the intensive culture of pikeperch larvae. Last two chapters (8 and 9) are describing the adaptation of intensively cultured juveniles to pond culture and the adaptation of pond-cultured juveniles to RAS as a new and effective methods for ongrowing production of pikeperch. In total, four published scientific papers, one handbook, one accepted scientific paper for publication, and two prepared scientific manuscripts are included and discussed in this Ph.D. thesis.
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Importance of the presence of parents for the eggs development and fry mortality in fish of the genus \kur{Hemichromis}
MICHALIČKA, Petr
Through this experiment there was examined the effect of the presence of the parents in the development and mortality of fish spawn of the genus Hemichromis. Hemichromis guttatus was selected for this experiment as a model species. It was found that the parental care in the fish genus Hemichromis is most needed during the period when the offspring is in the stage of eggs. In this stage, the absence of parents showed higher level of mortality (greater number of attacked by mold eggs left without parental care). In other developmental stages of the offspring the parental care was not a great advantage, on the contrary, in some cases allowing parents to take care of the screed was a disadvantage due to cannibalism.
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Using Persteril\copyright{} in practice for the prevention of fungal infections of eggs and fry baths sturgeon compared to commonly used products
HUDEC, Oldřich
Using Persteril36 (Acidum peraceticum) in practice for the prevention of fungal infections of eggs and fry baths sturgeon in comparison with the used products deals with the disinfection efficiency. In the experimental part were used three concentrations Persteril36, whose active ingredient is peracetic acid (36%), and the other were evaluated ToruMin, Acriflavin, TeraP and AquahumTM. Persteril36 in high concentrations appear to be very effective biocide, that leaves no residue and is not harmful to the environment. Other disinfectants have been evaluate detail in the results. Some of them appear to be very effective and they results exceeded expectations.
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Optimization of artificial spawning of pikeperch (Sander lucioperca) using HCG and new ways of removing artificial stickiness of eggs before incubation.
BLECHA, Miroslav
The object of the thesis is to experimentally find the most suitable method of artificial spawning of pikeprech, using hormonal stimulation of ovulation in females hormonal medication containing HCG. After scraping of the broodstock and after artificial fertilization of eggs obtained experimentally test new methods of artificial stickiness removing of eggs and then evaluate the success of artificial incubation of stripped, fertilized and unstickeness eggs of pikeprech. Experiments that are part of the thesis have been divided into several sub-experiments. In the first experiment was observed effect of different doses of hormonal Chorulon, containing the active substance HCG, the basic indicators of reproductive females. Was evaluated as spawning synchronization, spawning success, the number of spontaneous spawnings, fertility of females and hatching rate of eggs. The used HCG doses were 250, 500, 700 and 1000 IU per kilogram of body weight. All eggs were deprived of stickiness using a mixture of talc and milk. In the second experiment was carried out comparing different types of removes stickiness solutions. Here was evaluated especially time and labor intensity and the influence of various solutions on fertilization and hatching rate of eggs. In this trial was all pikeperch females injected the same amount of HCG hormone 500 IU per kilogram of body weight. Tested solutions was a mixture of talc and milk, just eggs wash water and the solution of various concentrations alkalase. The amount alkalase was 0,5; 1; 1,5; 2 and 5 ml, which are mixed with distilled water, so that the total volume of the solution was 1000 ml. The third experiment was supposed to simulate the pilot plant mass propagation of pikeperch females. All females received the same amount of hormone 500 IU of HCG per kilogram of body weight. All eggs were deprived of stickiness using a mixture of talc and milk. In the last experiment was one of the methods tested, which could limit the number of spontaneous spawning during the artificial spawning of pikeperch. To prevent spontaneous spawning were selected females (all were injected with 500 IU of HCG per kilogram of body weight) sewing genitourinary papilla. The incubation of eggs collected during all the experiments were used Zugské bottle.
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The Tolerance of Selected Species Fish Eggs to the Antifungal Baths
RAŠKA, Karel
The antifungal detergents Jodisol and Aquahum were tested on the fish eggs of the common carp (Cyprinus carpio), the sheatfish (Silurus glanis), the tench (Tinca tinca) and the common barbel (Barbus barbus). On the fish eggs of the African sharptooth catfish (Clarias gariepinus) the detergents Aquahum and Primrose were tested. The fish eggs from artificial spawn were used for the experiments. These experiments were running under laboratory conditions. The bath length was 5 minutes. The fish eggs incubation proceeded on laboratory dish at average water temperature, which was for the carp 22.87 ? 1.38 (min. 20.5; max. 23.8) °C, for the sheatfish 24.03 ? 2.12 (min. 20.4; max. 25.5) °C, for the tench 20.40 ? 2.13 (min. 18.0; max. 22.5) °C, for the common barbel 16.8 ? 0.95 (min. 14.5; max. 18) °C, and for the African sharptooth catfish 22.50 ? 0.35 (min. 22.0; max. 23.0) °C. Using Jodisol the best hatching success for the common carp was achieved when the concentration was 1 ? 10 ml?l-1, for the sheatfish 1 ml?l-1, for the tench 20 ml?l-1, and for the common barbel 50 ml?l-1. Using Aquahum the best hatching success for the common carp was achieved when the concentration was 1 ml?l-1, for the sheatfish 0.1 ml?l-1, for the tench 2 ml?l-1, and for the common barbel 0.1 and 2 ml?l-1. The stable preparation Aquahum with concentration 0.05 ml?l-1 proved the best results for the sheatfish. The preparation Primrose used for the African sharptooth catfish proved the best results with concentration 0.0033 and 0.01 ml?l-1. The detergent Aquahum could be recommended for the baths of the common barbel and the common carp. Jodisol worked well for the tench and the sheatfish. The best detergent for the African sharptooth catfish was Primrose.
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Different ways of reproduction of laboratory fish Danio rerio
KOMENDOVÁ, Jana
Danio rerio is often used in laboratories all over the world in varieties of fields. The demand for this fish is the precondition for building up a perfect system of its reproduction. The aim of the work was to find out the most suitable rate between the sexes of Danio rerio used for reproduction. The experiments were carried out on seven rates of sexes who represented the differences between big and small shoals. Males and females were rear separately. Fish in specific rates were used to spawn. The spawn were counted. Big shoals with one female staned in the spawn aquarium for one day. Little shoals for two days, the big ones for three days. The results were put in graphs. The most successful spawn was considered the one with the rate of one male to one female. Big shoals didn´t appear to be economic. The big number of fish had negative influence on the number of the spawn of one female of the shoal.
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